LSM 900 with Airyscan 2

Your Compact Confocal for Multiplex Imaging

What are you looking for in confocal imaging? Whatever your scientific question, you want to start with the best possible image quality and that means crisp contrast and the best resolution. You also want the highest sensitivity for gently imaging your living or fixed samples without bleaching. Your confocal LSM 900 with Airyscan 2 has all this and more. You image with 4 – 8× more signal-to-noise ratio (SNR) and with superresolution. You also get the highest frame rates: the new Multiplex mode for Airyscan 2 adds smart detection schemes for parallel pixel acquisition. You can now observe dynamic processes in living specimens gently – without sacrificing image quality. Plus, your LSM 900 has a genuinely small footprint, concentrating on the essence of a confocal and leaving off needless complexity. It fits easily into your lab or imaging facility – and it’s easy to use, too.

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Description

Highlights

  • Get Better Data Faster

Combine the excellent image quality of your confocal LSM 900 with the new Multiplex mode for Airyscan 2 to get more information in shorter times. You can now employ smart detection schemes to image your challenging three-dimensional samples with the highest framerates and with increased resolution of 140 nm. The speed and gentleness of the sensitive Airyscan area detector complement this compact point scanning confocal and allow you to image your most demanding samples with 4 – 8× more SNR.

HeLa cells stained for DNA (blue, Hoechst 44432), microtubules (green, anti-tubulin, alpaca anti-mouse

HeLa cells stained for DNA (blue, Hoechst 44432), microtubules (green, anti-tubulin, alpaca anti-mouse-alexa 488) and F-actin (red, phalloidin-Abberior STAR Red). Acquired with Multiplex mode for ZEISS Airyscan. © Courtesy of A. Politi, J. Jakobi and P. Lenart, MPI for Biophysical chemistry, Göttingen, Germany.

  • Increase Your Productivity

Your LSM 900 with Airyscan 2 is not just a compact confocal – it’s also very easy to use. Setup is simple with ZEN imaging software, even for complex confocal live cell imaging experiments. A wealth of software helpers lighten your workload and make sure you get reproducible results in the shortest possible time. Smart Setup and the new Sample Navigator let you find regions of interest and image them quickly, leaving you more time to acquire data. Direct Processing allows parallel acquisition and data processing. ZEN Connect keeps you on top of things at all times, both during imaging and later when sharing the whole story of your experiment. It’s easy to overlay and organize images from any source.

See how ZEN Connect helps to always keep your context while imaging. From acquiring an overview image, to defining ROI’s, and
even when changing between different imaging systems. You save time and always stay on top of things.

  • A Small Footprint for Greater Image Quality

Your LSM 900 is packed with innovative and clever solutions for producing the best quality in confocal live cell imaging. The elegant beam path is designed for high spectral flexibility, with each single component optimized for the highest sensitivity and contrast. Given its small footprint and reduced complexity, you’ll save valuable lab space, minimize the time needed for user-training and reduce the cost of ownership – this is especially good news for imaging facilities.

Cell division of LLC-PK1 cells, alpha-tubulin (mEmerald, magenta) and H2B (mCherry, green). With the new Multiplex mode for ZEISS Airyscan a Z-stack of 52 slices was captured every 40 seconds for a total of 40 minutes.

A Streamlined Light Path

With Surprising Flexibility

The compact light path with a minimum of optical elements is designed for highest efficiency. Fluorescence emission light travels through the main dichroic beam splitter with its outstanding laser suppression to deliver supreme contrast. Up to two patented variable beam splitter dichroics (VSDs) divert the spectral portions of emission light. You can define up to three detectors – multialkali, GaAsP or Airyscan 2. GaAsP PMTs – that is, gallium arsenide phosphide photomultiplier tubes – display high light collection efficiencies over a broad spectral range. Their low dark noise levels also render them the ideal tool for detecting faint signals.

Enjoy outstanding image quality based on a superb signal-to-noise ratio (SNR). You might use this gain in SNR to increase productivity by achieving faster scan speeds while preserving excellent image quality. Or take advantage of the low laser powers needed in confocal live cell imaging applications to avoid photobleaching and phototoxicity. Or simply detect faint signals in low expressing cells. All that, and you can do it with up to three spectral confocal channels

The Airyscan Principle

Principle of airyscan

Classic confocal laser scanning microscopes use point illumination to scan the sample sequentially. The microscope optics transform each point to an extended Airy disk (Airy pattern). A pinhole then spatially limits this Airy disk to block out-of-focus light from reaching the detector. Closing the pinhole gives higher resolution, but at the price of detecting fewer photons – and these photons cannot be brought back by e.g. deconvolution.

Airyscan 2 is an area detector with 32 concentrically arranged detection elements. This allows you to acquire more of the Airy disk at once. The confocal pinhole itself remains open and does not block light, thus more photons are collected. This produces much greater light efficiency while imaging. Airyscan 2 gives you a unique combination of gentle superresolution imaging and high sensitivity.

How the New Multiplex Mode for Airyscan 2 Works

The LSM 9 family with Airyscan 2 from ZEISS now gives you more options to fit imaging speeds and resolution to your experimental needs. You combine a confocal area detector with smart illumination and readout schemes, which let you choose from different parallelization options. The new Multiplex mode uses knowledge about the shape of the excitation laser spot and the location of single area detector elements to extract more spatial information, even during parallel pixel readout.

This allows taking bigger steps when sweeping the excitation laser over the field of view, improving your achievable acquisition speeds. In fact, the high amount of spatial information captured in the pinhole plane allows reconstructing a final image with better resolution than the acquisition sampling. Airyscan 2 in Multiplex mode can acquire up to four superresolution image lines with high SNR in a single sweep.

LSM 900 Airyscan SR Multiplex SR-2Y Multiplex SR-4Y Multiplex CO-2Y
Parallelization 1 2 4 2
Resolution 120/120 140/140 140/140 Confocal or better
Max. fps at max. field of view 0.4 0.8 3.5 3.5
Antibody labelling, fine structures +++++ ++++ ++++ ++
Antibody labelling, tiling ++ +++ +++++ +++
Live cell imaging ++ +++ ++++ +++++
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